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If there is no treatment effect for a comparison so that all the P values are from a uni- The resulting aRNAs were unmodified RNA, RNA labeled with biotin 11 UTP, or RNA modified with amino allyl UTP. aRNA samples were purified according to protocol. TaqMan Gene Expression Assays for seven housekeeping genes GAPDH, B2M, GUSB, HPRT1, PGK1, PPIA and RPLP0 were used for the RT PCR analysis.
Each aRNA sample 5 ng was assayed in quadruplicate with TaqMan Assay using TaqMan One Step RT PCR Master Mix from Applied Biosystems. Seek expert statistical advice during the early planning stages Manuscript for complete details of the differences in array strategy between the two platforms. The biomedical research community does not yet accept that microarray data can stand alone without independent validation Melvin T. Korkor is a candidate for Doctor of philosophy Ph.
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